THE DETECTION OF BACTEREMIA USING GRAM STAINING AND FLUORESCENT IN SITU HYBRIDIZATION IN SEPTIC PATIENTS WITH NEGATIVE BLOOD CULTURES

TOPIC: Critical Care TYPE: Original Investigations PURPOSE: The goal of the study was to assess if screening methods Gram staining and Fluorescent Hybridization In Situ (FISH) can increase rate detection bacteria in blood patients with negative culture results. METHODS: Blood cultures 2 ml-blood samples fulfilling criteria sepsis were analyzed. underwent standard testing automated systems. results subjected erythrocytes lysis (creating a strong background) according methodology described by Gosiewski et al. [patent: US9879311 (B2)]. remaining sediment used prepare microscope slides for FISH. preparations analyzed using light BX51 (Olympus) fluorescence respectively. RESULTS: total, 143 demonstrated growth 15.38% (n=22).Remaining 121 obtained from tested use staining, visualized 51.24% (n=62), FISH detected 61.98% (n=75). time required obtain test 1 hour 4 hours CONCLUSIONS: implemented allows directly collected septic FISH.The confirms effectiveness both microbiological whole blood, but demonstrates greater sensitivity when compared staining. are inexpensive, easily available that provide timely manner. They could be as quick diagnostics bacteremia. CLINICAL IMPLICATIONS: bacteremia consists culture. Although culture-positive is found about 30% shock, its presence associated higher mortality rates. advantage simplicity cost effectiveness. disadvantage this method time-consuming has low sensitivity. Time needed initiate targeted therapy direct impact on patient's survival. Molecular based genetic analysis costly currently only select number research centers. Therefore, new quickly reveal would extremely useful. DISCLOSURES: No relevant relationships Tomasz Gosiewski, source=Web Response Isabel McFarlane, Dominika Salamon, source=Admin input Joanna Sobonska, Miroslaw Ziętkiewicz, ZRODLOWSKI,